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Edu-talk

이매스교육 및 공지사항

Edu-talk

  • 제목

    [MS Basics] 일반 ESI Source Ion Spray Guide
  • 작성자

    이매스
  • 첨부파일

 추천버퍼조건(Positive 모드)
Ammonium formate or acetate (2-10 mM optimum, can see suppression effects over 20 mM).
Acetic acid at 0.1 - 1.0 %
0.1% TFA (good for chromatography)에서 Formic acid 는 약 0.1% 가 적당함
 
Probe 온도조건
대부분의 분석물질은 약 150°C 가 한계.
Solvent flow rate 에 따라 최저와 최고 온도를 적절하게 조절함.
 
Compounds That Cause Sensitivity Suppression
Salts can interfere with ionization and can cluster to complicate spectrum (but also aid in identification).
Strong bases or quaternary amines can interfere with positive mode analytes, e.g. Triethylamine (TEA)
Acids - Sulfuric/Sulfonic acids and TFA interfere in negative mode experiments.
Phosphate buffer and non-volatile ion pairing agents (e.g. SDS) can cause severe suppression and complex spectra.
Dimerization ([2M+H]+) can occur at high concentration, leading to non-linearity during Quantitation.
Dimer Signal at m/z = (MW*2)+1 can cause non-linearity at high concentrations.