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제목
[Proteomics] Protein Sequencing 의 활용예 - Human Growth Hormone -
작성자
이매스 -
첨부파일
Edman Sequecing 의 검증된 활용예
제목 : The ABRF Edman Sequencing Research Group 2008 Study: Investigation into Homopolymeric Amino Acid N-Terminal Sequence Tags and Their Effects on Automated Edman Degradation
저자 : R. S. Thoma, J. S. Smith, W. Sandoval, J. W. Leone, P. Hunziker, B. Hampton, K. D. Linse, and N. D. Denslow
재조합 Human Growth Hormone 의 발현에있어 N 말단 분석을 활용한 다양한 poly Tag 의 분석에 관한 활용기법을 소개한 글을 여러분께 소개할까 합니다.
Test Proteins
Each of N-terminally tagged proteins in this study consisted of 11 aa preceding hGH.
The tag incorporated a string of 8 aa polyhistidine or polyalanine for the two test proteins.
DNA construct preparation was simplified by the addition of extra nucleotides that
added 3 aa to the tag portion of the two proteins, which were designed so that
the N-terminal amino acid of hGH begins at cycle 12. The control protein was untagged hGH.
The tag incorporated a string of 8 aa polyhistidine or polyalanine for the two test proteins.
DNA construct preparation was simplified by the addition of extra nucleotides that
added 3 aa to the tag portion of the two proteins, which were designed so that
the N-terminal amino acid of hGH begins at cycle 12. The control protein was untagged hGH.